时    间：2023年6月7日16:00

地    点：实验教学主楼822会议室

主 持 人：高灿 教授

报 告 人：胡静 副教授

题    目：单细胞转录组学在探究肿瘤免疫治疗新靶点与新机制中的应用案例----MK801通过抑制肝癌微环境中巨噬细胞NMDAR的抗肿瘤免疫作用机制

主要内容：

     The expression of the ion channel N-methyl-D-aspartate receptor (NMDAR) on macrophages was reported, but the role of NMDAR on macrophages in the tumor microenvironment (TME) remains unknown. Here, we show that the activation of NMDAR triggered calcium influx and ROS production, which fueled immunosuppressive activities in tumor-associated macrophages (TAMs). NMDAR channel blockers, MK-801 effectively suppressed these processes in TAMs. mRNA sequencing and Single-cell RNA-sequencing analysis revealed that blocking NMDAR functionally and metabolically altered TAM phenotypes, such that they could better promote T cell- and NK cell-mediated anti-tumor immunity. Treatment with NMDAR channel blockers in combination with anti-PD-1 antibody led to elimination of tumor.